【 Principle 】

Peptone provides nitrogen sources, peptides, and amino acids to meet the basic growth needs of heterotrophic bacteria; Yeast extract powder supplements B vitamins, trace elements, and various growth factors to enhance bacterial activity and ensure normal expression of enzyme systems; Potassium nitrate: As the core substrate of this experiment, it dissociates and releases nitrate ions, which are used to detect whether bacteria synthesize nitrate reductase.

Main components

Composed of peptone, yeast extract powder, potassium nitrate, and distilled water.

Storage conditions and expiration date

Storage conditions: This product should be stored in a dark environment at 2-8 ℃.

Validity period: The unopened original packaging has a validity period of 6 months.

This product is a disposable product.

Biochemical identification of bacterial nitrate reduction: Biochemical typing of pathogenic bacteria such as Enterobacteriaceae, Pseudomonas, and Corynebacterium, distinguishing nitrite positive, nitrogen producing positive, and non nitrate reducing strains.

Assisted identification of hospital isolated bacterial strains: Biochemical tests for clinical sputum, urine, pus, and blood isolation of pure bacteria to assist in the identification of common infectious bacteria such as Pseudomonas aeruginosa and Escherichia coli.

Testing of drugs and water source indicator bacteria: Biochemical confirmation test of Gram negative bacteria isolated from purified water and raw materials, improving the microbial identification process.

Verification of biochemical characteristics of quality control strains: During the passage and acceptance of standard strains, the nitrate reduction reaction is rechecked to ensure the effectiveness of the experimental culture medium.

Screening and identification of environmental pathogenic bacteria: biochemical typing of strains isolated from hospital countertops, equipment, and wastewater, used for distinguishing strains of nosocomial infections.

Escherichia coli

Gram negative short rod-shaped bacteria, with flagella, motility, no spores, facultative anaerobic; Stable reduction of nitrate to nitrite; Widely present in the human gut, water sources, food, and medical environment, it is a hygiene indicator bacterium and a common opportunistic pathogen.

Salmonella genus

Gram negative bacteria, with flagella around the body, active motility, no spores, facultative anaerobic; Positive nitrate reduction test; Typical intestinal pathogenic bacteria can cause acute gastrointestinal infections.

Shigella genus

Gram negative bacteria, flagellar free, motile, spore free, facultative anaerobic; Capable of nitrate reduction; Specialized intestinal pathogenic bacteria lead to bacterial dysentery.

Klebsiella and Escherichia genera

Gram negative crude short rod-shaped bacteria, most of which have capsules, lack motility, and are facultative anaerobic; Nitrate reduction positive; Commonly found in hospital environments, medical equipment, and purified water, it is a common pathogenic bacterium for nosocomial infections.

Pseudomonas aeruginosa

Gram negative filamentous bacteria, single ended flagella, obligate aerobic; Production of pyocyanin; Strong reducing ability, further decomposition of nitrite into N2, visible bubbles in the inverted tube; Wound, respiratory, and instrument infections are common.