【 Principle 】

Tryptone and yeast extract powder provide carbon and nitrogen sources, vitamins, and other nutrients; Glucose provides fermentable sugars that are more conducive to growth; Sodium chloride maintains a balanced osmotic pressure; Sodium thioglycolate and L-cysteine can effectively reduce the oxidation-reduction potential, prevent the accumulation of peroxides from causing toxicity to certain bacteria, and their thiol groups have the antibacterial effect of passivating preservatives containing arsenic, mercury, and other heavy metals; The coagulation effect of a small amount of agar can prevent the diffusion of carbon dioxide, oxygen, and reduction products; Blade Sky Blue is an oxidation-reduction indicator, with a pink oxidation state and colorless reduction state.

Main components

Composed of pancreatic casein peptone, yeast extract powder, glucose, sodium chloride, L-cysteine, sodium thioglycolate, agar, cresol, and distilled water.

Storage conditions and expiration date

Storage conditions: This product should be stored in a dark environment at 2-25 ℃.

Validity period: The unopened original packaging has a validity period of 6 months.

This product is a disposable product.

[Sample Requirements]

Samples such as blood and ascites from the human body.

Aseptic specimen mixed bacterial enrichment culture: simultaneous enrichment of aerobic, facultative anaerobic, and obligate anaerobic bacteria in pus, puncture fluid, and deep wound secretions to screen for mixed infection pathogens.

Aseptic inspection of drugs and medical devices: Pharmacopoeia standard bacterial enhancement solution can detect both aerobic and anaerobic pollutants at once to avoid false negatives caused by missed anaerobic bacteria.

Screening of anaerobic pathogenic bacteria in blood samples: Assist in detecting rare blood flow anaerobic pathogens such as Clostridium and anaerobic streptococcus, and improve the diagnosis of sepsis pathogens.

Pre inoculation of anaerobic bacteria before isolation: Improve the detection rate of trace anaerobic bacteria specimens, and subsequently obtain single colonies by transferring to solid agar plates.

Microbial risk monitoring for nosocomial infections: Sampling of surgical instruments, flushing fluids, and dressings, while screening for pathogenic bacteria under aerobic and anaerobic conditions.

Standard strain recovery and passage: Taking into account different types of oxygen metabolism strains, it is used for short-term activation and cultivation of aerobic and anaerobic strains.

Staphylococcus: Staphylococcus aureus, facultative anaerobic (aerobic growth is better), with strong resistance; A thin milky white bacterial film forms on the liquid surface, with clear liquid in the lower layer and a small amount of fine white sediment at the bottom.

Bacillus subtilis (Bacillus subtilis, Bacillus cereus): G ⁺ aerobic Bacillus subtilis, producing spores, high temperature resistance, disinfectant; The liquid surface is thick with gray white wrinkled bacterial membrane, the bottom layer is clear, and foam can be seen from intense gas producing strains.

Pseudomonas aeruginosa: a G ⁻ - specific aerobic bacterium that prefers moisture and produces water-soluble green pigments; Thin gray green bacterial film on the liquid surface, the overall culture medium turns green, and the bottom layer is clear

Enterobacteriaceae (Escherichia coli, Klebsiella, Salmonella): Gram negative bacteria that can rapidly reproduce both aerobic and anaerobic conditions, mostly intestinal/opportunistic pathogens; The entire tube is uniformly turbid, with no obvious surface bacterial film, and there is a grayish white sediment at the bottom.