【 Principle 】

Animal tissue hydrolysates provide peptides, amino acids, nitrogen sources, vitamins, and growth factors; Corn starch adsorbs trace amounts of toxic oxygen free radicals and heavy metal impurities in the culture medium, protecting fragile and harsh bacteria while buffering pH stability; Sodium chloride maintains the osmotic pressure of the culture medium; Agar is a coagulant; Aseptic defibrated sheep blood provides hemoglobin, growth factors, and red blood cell matrix, supporting the growth of harsh bacteria, assisting in the identification of hemolytic reactions, and neutralizing oxidative substances to protect damaged bacterial cells.

Main components

It is composed of animal tissue hydrolysate, corn starch, sodium chloride, agar, sterile microfiber sheep blood, and distilled water.

Storage conditions and expiration date

Storage conditions: This product should be stored in a dark environment at 2-8 ℃.

Validity period: The unopened original packaging has a validity period of 3 months.

This product is a disposable product.

[Sample Requirements]

Samples such as sputum, blood, urine, cerebrospinal fluid, and wound secretions from the human body.

Primary isolation of clinical specimens: suitable for blood, pus, sputum, cerebrospinal fluid and other specimens, and can be used to culture common bacteria and harsh culture bacteria.

Observation of Hemolysis Characteristics: Rapid identification of Group A Streptococcus, Streptococcus pneumoniae, etc. through alpha, beta, and gamma hemolysis to assist in initial screening of bacterial strains.

Purification of suspicious colonies: Select suspicious colonies from the selective plate and transfer them to obtain pure colonies for subsequent identification.

Recovery of harsh bacteria: Corn starch adsorbs oxygen toxicity, and sheep blood provides X/V factors to increase the detection rate of damaged harsh bacteria.

Preparation of drug sensitive bacterial suspension: Prepare standardized bacterial suspension after the purified strain is passaged and revived to ensure accurate drug sensitivity results.

Aseptic specimen infection screening: Cultivate sterile body fluids such as cerebrospinal fluid and blood to screen for systemic infection related pathogens.

Special testing kit: in combination with selective culture media such as Campylobacter and Listeria, used for strain purification and hemolytic phenotype confirmation.

Streptococcus pyogenes

Biological characteristics: Gram positive Streptococcus, facultative anaerobic; Producing hemolysin O/S can cause diseases such as pharyngitis, cellulitis, and sepsis.

Cultivation characteristics: At 35 ℃ for 24 hours, small gray white raised colonies with clear and transparent beta hemolytic rings around the periphery of the colonies; Enzyme negative.

Streptococcus pneumoniae

Biological characteristics: Gram positive diplococcus, arranged in pairs in a pointed shape, with capsules; Alpha hemolysis can cause pneumonia, meningitis, and otitis media.

Cultivation characteristics: Flat, semi transparent needle like colonies surrounded by grass green alpha hemolytic rings; Bile lysate is positive.

Listeria

Biological characteristics: Gram positive Bacillus subtilis, motile at 25 ℃, a zoonotic pathogen that can cause sepsis and meningitis.

Cultivation characteristics: small blue gray colonies, narrow beta hemolytic ring; 4 ℃ can increase bacterial growth by cold treatment, and the contact enzyme is positive.

Staphylococcus (Staphylococcus aureus, coagulase negative Staphylococcus)

Biological characteristics: Gram positive Staphylococcus aureus, facultative anaerobic; Staphylococcus aureus produces various toxins, causing skin infections and bloodstream infections.

Cultivation characteristics: large golden/milky white colonies; Staphylococcus aureus shows a wide beta hemolytic ring, while Staphylococcus epidermidis is mostly gamma hemolytic (without hemolysis) and positive for lysozyme.

Haemophilus influenzae (Haemophilus influenzae, etc.)

Biological characteristics: Gram negative short ball bacillus, strictly requires X and V factors, belongs to the category of harsh bacteria, causing respiratory and meningeal infections.

Cultivation characteristics: transparent colonies resembling small dewdrops, without hemolysis; It relies on heme and coenzyme in sheep blood to grow.