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Nutrient Agar
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Product Code:PD-030102056
Product Specifications:

150mm120mm100mm90mm70mm60mm55mm35mm

Product Usage:适用于非苛养微生物的常规培养、分离、纯化与菌落计数。
Product Overview

【 Principle of Inspection 】

Peptone and beef powder provide nitrogen, vitamins, amino acids, and carbon sources, while sodium chloride can maintain a balanced osmotic pressure. Agar is the coagulant of the culture medium. Main components

Composed of tryptone, beef extract powder, sodium chloride, and agar.

Storage conditions and expiration date

Storage conditions: This product should be stored in a dark environment at 2-8 ℃.

Validity period: The unopened original packaging has a validity period of 6 months.

This product is a disposable product.


Clinical application scenarios

Bacterial count of specimens: determination of the total number of bacteria in sewage, drinking water, medical equipment, environmental surfaces, and disinfection effectiveness monitoring;

Purification and passage of bacterial strains: Purification, enrichment, and preservation of common pathogenic bacteria (such as Escherichia coli, Staphylococcus aureus, Bacillus subtilis, etc.) isolated clinically;

Pure bacterial biochemical pre culture: Select suspicious colonies from selective agar plates, transfer them to nutrient agar for pure culture, and perform biochemical identification;

Pre sensitization bacterial enhancement: pure cultivation of non harsh bacteria, used for preparing bacterial solution for paper susceptibility testing;

Quality control comparison: Microbial laboratory culture medium performance, strain recovery, and daily quality control cultivation.




Introduction to the Relevant Microbiological Characteristics

Staphylococcus aureus: Gram positive cocci, typically arranged in "grape clusters", without spores or flagella, and some strains can form capsules; Rapidly growing on nutrient agar, forming round, raised, smooth and moist colonies on the surface; The diameter is about 2-3mm, with a typical golden yellow color. Transparent hemolytic rings may appear around the colony, and there is no special odor. It is enzyme positive and can decompose glucose, maltose, and other substances to produce acid and gas, but does not decompose lactose; It can produce plasma coagulase, has strong tolerance to dryness and salt, and can still grow in an environment of 10% to 15% sodium chloride.

Escherichia coli: Gram negative short rod-shaped bacteria without spores and with multiple flagellar pili. It is facultative anaerobic and grows into milky white smooth medium-sized colonies on nutrient agar. It can ferment glucose and lactose to produce acid and gas, and has an IMViC of++- - -. It contains O/H/K antigens, is not resistant to high fever, and is easily inactivated by conventional disinfectants. Most of them are normal gut microbiota, and pathogenic subtypes can cause intestinal and extra intestinal infections.