Preliminary screening of coliform bacteria in food water samples: a national standard core reagent used for initial fermentation of coliform bacteria in water samples, fresh food, and tableware smear samples.

Distinguishing Lactose Fermented Enterobacteriaceae: Quickly identify the Escherichia coli population through gas and acid production, and exclude non fermenting bacteria such as Salmonella and Proteus.

Monitoring of water source hygiene and safety: Microbial screening of tap water, bottled water, sewage, and surface water to determine fecal pollution in water bodies.

Fecal contamination indicator bacteria screening: using coliform bacteria as a fecal contamination indicator, indirectly reflecting the risk of intestinal pathogenic bacteria contamination.

MPN method for quantitative counting: gradient dilution inoculation, estimating the most likely number of coliforms through a positive tube count lookup table.

Confirmation of initial fermentation positive diversion: Transfer of gas and acid producing positive tubes to EMB and BGLB for confirmation of coliform bacteria.

Clinical fecal assisted screening: assists in distinguishing between lactose fermenting pathogenic bacteria and non fermenting intestinal pathogenic bacteria, supporting initial screening for intestinal infections.

【 Principle 】

Peptone provides ammonia and nitrogen sources to meet the needs of bacterial growth; Pig bile salt can inhibit the growth of Gram positive bacteria; Saccharides that can be fermented by lactose rich Escherichia coli; Bromocresol purple is a pH indicator, with acidity appearing yellow and alkalinity appearing purple.

Main components

Composed of peptone, pig bile salt, lactose, bromocresol violet, and distilled water.

Storage conditions and expiration date

Storage conditions: This product should be stored in a dark environment at 2-8 ℃.

Validity period: The unopened original packaging has a validity period of 3 months.

This product is a disposable product.

Escherichia coli

Biological characteristics: Gram negative short rod-shaped bacteria, approximately 0.5 μ m x 1-3 μ m in size; Facultative anaerobic, with flagella all over the body and active movement; Fermentation of lactose to produce acid and gas; Oxidase negative, indole positive; The growth temperature is 35-37 ℃.

Cultivation characteristics: Cultivate at 35-37 ℃ for 18-24h, and the broth appears uniformly turbid; Bromocresol purple indicator changes from purple to yellow; The gas inside the Du's small tube fills more than one-third of the tube.

klebsiella pneumoniae 

Biological characteristics: Gram negative short rod-shaped bacteria, flagellar free and non motile; It has a thick capsule and the bacterial cells are oval in shape; Fermentation of lactose to produce acid and gas; Facultative anaerobic, with low nutritional requirements; Conditional pathogenic bacteria can cause pneumonia, urinary tract infections, etc.

Cultivation characteristics: 35-37 ℃, 18-24h cultivation, uniform turbidity of broth; Bromocresol purple indicator yellow; Du's tubules produce gas (slower, some strains require extended cultivation).

Enterobacter aerogenes

Biological characteristics: Gram negative bacteria with flagella and active movement; Facultative anaerobic; Fermentation of lactose to produce acid and gas; VP test positive.

Cultivation characteristics: 35-37 ℃, 24-hour cultivation, uniform turbidity of broth; Bromocresol purple indicator yellow; Du's tubules produce gas (with significant gas production).