【 Principle 】

Tryptone and beef extract provide carbon and nitrogen sources, vitamins, and growth factors; Glucose provides energy; Sodium chloride maintains a balanced osmotic pressure; Agar is a coagulant for culture media; Clostridium perfringens has the ability to break down phospholipids into insoluble glycerides using lecithin enzymes, resulting in a milky white cloudy band.

Main components

Composed of tryptone, beef extract, glucose, sodium chloride, yolk, agar, and distilled water.

Storage conditions and expiration date

Storage conditions: This product should be stored in a dark environment at 2-8 ℃.

Validity period: The unopened original packaging has a validity period of 3 months.

This product is a disposable product.

[Sample Requirements]

Samples of abdominal fluid, joint fluid, etc. from the human body.

Isolation of Clostridium from Trauma Specimens: Anaerobic culture was performed on deep wounds and gas gangrene wounds, and Clostridium perfringens was detected by lecithin enzyme white ring, confirming the diagnosis of gas gangrene; Lipase halo screening for botulism/Clostridium difficile.

Traceability of food poisoning: spoiled meat products, patient fecal culture, Clostridium perfringens indicates acute food poisoning, and Clostridium botulinum is screened for botulism.

Purification and identification of bacterial strains: Select characteristic bacterial colonies for purification, assist in biochemical typing of clostridia, and provide pure bacteria for toxin detection and drug sensitivity.

Necrotic enteritis detection: isolation of Clostridium perfringens (type C) from infants/severe bloody feces to assist in the diagnosis of necrotic enteritis.

Research and Quality Control: Propagation and Preservation of Standard Clostridium Strains and Laboratory Method Quality Control.

Clostridium perfringens

Form: G ⁺ Coarse Bacillus, spores located at the sub extreme, obligate anaerobic, with capsules.

Colony: A milky white opaque phospholipid precipitation ring (positive for lecithin enzyme) appears around the colony, surrounding it; Some strains are lipase negative.

Pathogenesis: gas gangrene, food poisoning, necrotic enteritis.

Clostridium botulinum

Biological characteristics: Gram positive Bacillus subtilis, spindle shaped, with flagella that can move slowly, without capsule; It can form oval shaped spores, located at the second extreme of the bacterial body, with a width larger than the bacterial body, giving it extremely strong heat resistance; Strictly anaerobic bacteria, suitable for growth at a temperature of about 35 ℃; Pathogenicity arises from the production of botulinum toxin, which is a neurotoxin.

Colony morphology: almost circular, with irregular edges, a diameter of about 3mm, protruding from the surface of the plate, appearing gray white, smooth or granular on the surface; Lipase activity (iridescence phenomenon), with a pearl like luster around the colony under oblique light irradiation (except for G-type); Phosphatidyltransferase activity leads to the formation of a milky ring at the bottom and around the colony of some strains.