Sterilized Polysorbate 80 is a high-purity, nonionic surfactant processed under aseptic conditions. Widely used in biopharmaceuticals and sterile preparations, it serves as a critical pharmaceutical excipient. Its primary applications include enhancing solubility, emulsification, and protein stability in injectables, vaccines, and biomolecular drugs. It effectively improves the solubility of lipophilic components, inhibits protein aggregation and interfacial adsorption during storage and transportation, and is utilized in microbial culture media (e.g., Middlebrook 7H9) to prevent microbial contamination. emulsifying, and protein stabilizing functions in injectables, vaccines, and biomolecular drugs. It effectively enhances the solubility of lipophilic components, inhibits protein aggregation and interfacial adsorption during storage and transportation, and promotes the dispersion and utilization of poorly soluble nutrients in microbial culture media (e.g., Middlebrook 7H9). Additionally, it neutralizes residual preservatives such as phenols to safeguard microbial growth environments.

Neutralizing disinfectant residues: In environmental monitoring and sterility testing, neutralize the residues of quaternary ammonium salts, iodine, phenols, hexachlorophenol, formalin and other disinfectants on the sampling surface to avoid false negative results.

Microbial limit test for cosmetics and drugs: added to the culture medium to neutralize preservatives in the sample, used for counting the total number of aerobic bacteria in products containing antibacterial ingredients.

Removing the antibacterial effect of drugs: In the sterile inspection of injection and other drugs, adding culture medium or rinsing solution can effectively remove the antibacterial effect of the sample itself, ensuring the normal growth of positive control bacteria.

Sample dilution and microbial elution: Prepare Tween-80 aqueous solution for dilution and elution of microorganisms in samples such as air conditioning system surfaces and equipment surfaces, and use it as a dispersant.

Promoting dispersed growth of microorganisms: In specific culture medium formulas (such as Mycobacterium tuberculosis culture medium), it can promote the dispersed growth of bacterial cells, which is convenient for subsequent experimental operations.