【 Principle 】

Tryptone provides the necessary nutrients for growth; Sodium chloride maintains a balanced osmotic pressure.

Main components

Composed of tryptone, sodium chloride, and distilled water.

Storage conditions and expiration date

Storage conditions: This product should be stored in a dark environment at 2-25 ℃.

Validity period: The unopened original packaging has a validity period of 6 months.

This product is a disposable product.

[Sample Requirements]

Fecal samples from the human body.

Bacterial biochemical identification: detecting whether bacteria produce tryptophan enzyme, used to identify Enterobacteriaceae bacteria, is one of the key biochemical tests in clinical microbial identification.

Identification of intestinal pathogens: used to distinguish intestinal pathogens such as Escherichia coli (indole positive), Shigella (variable response), Salmonella (negative), etc.

Pre enrichment of Salmonella in Food: Buffered Peptone Water (BPW) is used for the recovery and pre enrichment of damaged Salmonella in food samples.

Pre enrichment of Cronobacter in Food: BPW is also suitable for pre enrichment culture of Escherichia coli (Cronobacter) in infant formula food.

Biochemical identification of Vibrio species: 1% NaCl peptone water was used for the identification of sodium chloride resistant Vibrio species through indigo test.

Fermentation experiment basic medium: Andrade peptone water can be used as a basic nutrient matrix, and after adding specific carbohydrates, it can be used for studying the fermentation characteristics of bacteria

Enterobacteriaceae bacteria (Escherichia coli, Proteus, Salmonella, Shigella, Klebsiella, etc.)

Most of them are Gram negative Bacillus subtilis without spores, facultative anaerobic, and have low nutritional requirements; Some strains contain tryptophan enzymes, which can decompose tryptophan in the culture medium to produce indole; The culture medium does not contain sugars, and bacteria only decompose peptone for growth, without fermentation to produce acid or gas;

After inoculation with bacteria, the culture medium became uniformly turbid, with a small amount of gray white bacterial sediment at the bottom of the tube. The liquid surface had a sterile membrane, and the culture medium did not produce gas or pigments; Indole positive: Rose red ring appears on the liquid surface (Escherichia coli, Proteus mirabilis); Indole negative: There is no red ring on the liquid surface, and the original solution remains light yellow (Klebsiella, Salmonella).