【 Principle of Inspection 】

Peptone and beef extract powder provide nitrogen sources, amino acids, vitamins, and inorganic salts, providing basic nutrients for bacterial growth; Gelatin, as the sole substrate, is used to detect the ability of bacteria to secrete gelatinase to liquefy it; Agar is a coagulant.

Main components

Composed of peptone, gelatin, beef extract powder, agar, and distilled water.

Storage conditions and expiration date

Storage conditions: This product should be stored in a dark environment at 2-8 ℃.

Validity period: The unopened original packaging has a validity period of 6 months.

This product is a disposable product.

Distinguishing similar bacterial species: using gelatin liquefaction test to identify different bacterial genera such as Proteus, Bacillus, Clostridium, etc.

Toxicity screening: Gelatinase positive strains have stronger invasion ability, which can assist in evaluating the pathogenic potential of Staphylococcus aureus, copper green and other bacteria.

Anaerobic bacteria identification: used for detecting anaerobic bacteria such as Clostridium perfringens, and assisting in the diagnosis of infections such as gas gangrene.

Microbial confirmation of drugs: In conjunction with other biochemical tests, confirm suspicious strains such as copper green, Staphylococcus aureus, and Bacillus cereus.

Environmental Bacillus testing: Distinguish Bacillus subtilis from Bacillus subtilis, wax like Bacillus subtilis, and thermophilic fat loving Bacillus subtilis for sterilization effectiveness verification.

Standard strain quality control: Verify whether the biochemical characteristics of laboratory standard strains are stable to prevent variation or confusion.

Staphylococcus aureus

Staphylococcus aureus, facultative anaerobic; Secreting various extracellular proteases, it can infect pathogenic bacteria in skin, wounds, and bloodstream; Cultivate at 37 ℃ and grow along the puncture line; After a few days, the gelatin around the puncture gradually liquefies; The colonies on the tablet are milky white/golden yellow, smooth and opaque.

Pseudomonas aeruginosa

G ⁻ obligate aerobic bacteria, opportunistic pathogens, secrete large amounts of extracellular proteases, elastase, and gelatinase; Fast liquefaction rate, large-scale liquefaction of the culture medium; The colony is flat, surrounded by agar that produces pyocyanin and has a ginger aroma.

Clostridium perfringens (anaerobic bacteria)

G ⁺ Anaerobic Bacillus subtilis, producing Clostridium perfringens toxin containing strong protease, causing gas gangrene; Under anaerobic conditions, gelatin completely liquefies, accompanied by gas production; Hemolysis and irregularity of flat bacterial colonies.

Bacillus subtilis, Bacillus cereus

G ⁺ Aerobic Bacillus subtilis is widely present in the environment and has extremely strong extracellular protease activity; Fully liquefy gelatin in a short period of time; The colony is rough, flat, gray white, and has irregular edges.