[Principle]

Peptone and beef extract provide amino acids, carbon sources, nitrogen sources, and vitamins, while agar serves as the solidifying agent.

[Main Ingredients]

Composed of peptone, beef extract, and agar.

[Storage Conditions and Shelf Life]

Storage conditions: This product should be stored in a light-protected environment at 2-8°C.

Validity period: 3 months when the original packaging remains unopened.

This product is for single-use only.

Strain passage and scale-up culture: The purified strain is inoculated onto slant culture media, and after incubation, a large quantity of fresh microbial biomass is obtained for subsequent biochemical identification, serological tests, antimicrobial susceptibility assays, and other purposes.

Short-term low-temperature preservation of microbial strains: Store the cultured slant cultures in a 4°C refrigerator. Bacteria can generally be preserved for 1-3 months, making it the simplest method for strain preservation in laboratories.

Salmonella H antigen phase variation induction: Utilize specific concentrations of nutrient agar slants to perform the Salmonella flagellar antigen phase variation test, achieving a high success rate in a single induction.

Observation of Strain Purity and Viability: After slant cultivation, the colony morphology, pigment production, and other characteristics can be directly observed, while regularly checking for contamination or mutation of the strain.

Blood agar slant base components: Add defibrinated sheep or rabbit blood to regular nutrient agar to prepare blood agar slants, which are used for isolating and culturing bacteria with higher nutritional requirements

Staphylococcus aureus: Gram positive cocci, arranged in clusters of grapes; Facultative anaerobic, without spores; The cultured colony appears yellow and grows vigorously.

Escherichia coli Gram negative short rod bacteria with flagella; Facultative anaerobic, representing the gut microbiota; The colony grows vigorously, forming colorless and transparent large colonies.

Pseudomonas aeruginosa: Gram negative bacteria, obligate aerobic; Widely present in the environment; The colony grows vigorously, and the colony and culture medium can present green pigment (pyocyanin).

Bacillus cereus: Gram positive bacillus, aerobic, capable of forming spores; The colony grows vigorously, forming white, irregular colonies.

Salmonella genus: Gram negative bacteria, facultative anaerobic; Intestinal pathogenic bacteria; The colony grows vigorously and forms colorless large colonies, which are similar in morphology to Escherichia coli and require further identification.

Listeria monocytogenes: Gram positive short rod-shaped bacterium, facultative anaerobic, can grow at low temperatures; The colony grows well and forms colorless and transparent small colonies.

Vibrio cholerae: Gram negative arc-shaped bacteria, facultative anaerobic; Requires alkaline environment for growth; The colony grows well on this medium (pH 8.0-8.2) and forms colorless colonies.