【 Principle 】

Tryptone and yeast extract powder provide carbon and nitrogen sources, vitamins, and other nutrients; Glucose provides fermentable sugars that are more conducive to growth; Sodium chloride maintains a balanced osmotic pressure; Sodium thioglycolate and L-cysteine can effectively reduce the oxidation-reduction potential, prevent the accumulation of peroxides from causing toxicity to certain bacteria, and their thiol groups have the antibacterial effect of passivating preservatives containing arsenic, mercury, and other heavy metals; The coagulation effect of a small amount of agar can prevent the diffusion of carbon dioxide, oxygen, and reduction products; Blade Sky Blue is an oxidation-reduction indicator, with a pink oxidation state and colorless reduction state.

Main components

Composed of pancreatic casein peptone, yeast extract powder, glucose, sodium chloride, L-cysteine, sodium thioglycolate, agar, cresol, and distilled water.

Storage conditions and expiration date

Storage conditions: This product should be stored in a dark environment at 2-25 ℃.

Validity period: The unopened original packaging has a validity period of 6 months.

This product is a disposable product.

[Sample Requirements]

Samples such as blood and ascites from the human body.

Aseptic inspection of drugs, injections, and medical devices : balancing aerobic, facultative anaerobic, and obligate anaerobic bacterial proliferation to determine the sterility of the sample.

Clinical specimen enrichment: integrated aerobic and anaerobic bacterial enrichment culture for pus, puncture fluid, and blood specimens.

Preliminary cultivation of anaerobic bacteria: The bottom anaerobic zone is dedicated to the growth of specialized anaerobic bacteria, while the upper aerobic layer is used to cultivate aerobic bacteria.

Recovery and preservation of bacterial strains: activation and passage of aerobic and anaerobic standard bacterial strains.

Microbial enrichment detection of samples neutralized with disinfectants.

Staphylococcus aureus: Staphylococcus aureus, without spore flagella, facultative anaerobic; The entire tube is uniformly turbid and positive for enzymes; Wounds, blood flow, and foodborne infections.

Pseudomonas aeruginosa: G. monocytogenes, obligate aerobic; Only grows on the surface layer, and the culture medium is yellow green with a ginger flavor; Pathogenic bacteria under hospital conditions.

Escherichia coli: G ⁻ - flagellar short rod-shaped bacteria, without spores; The entire tube is uniformly turbid, with positive results for fermented glucose and indole; Normal intestinal bacteria can cause urinary tract infections and enteritis.

Clostridium perfringens: G ⁺ crude bacillus, sub extreme spore, obligate anaerobic; Only grows in turbid conditions at the bottom of the pipe; Gas gangrene and food poisoning.

Fragile Bacteroidetes: G ⁻ polymorphic bacteria, non spore and non flagellate, obligate anaerobic; Bottom layer growth is turbid; The primary anaerobic bacteria are found in mixed infections of the abdominal cavity and deep pelvic cavity.