【 Principle 】

Hydrolyzed peptone, plant peptone, and yeast extract provide the nitrogen source, vitamins, and growth factors required for bacterial growth; Glucose is a fermentable sugar; Tween 80 and cysteine hydrochloride provide growth factors for various bifidobacteria in the culture medium; Potassium dihydrogen phosphate, zinc sulfate, magnesium chloride, calcium chloride, and ferric chloride provide trace elements.

Main components

Composed of hydrolyzed peptone, plant peptone, yeast extract, glucose, cysteine hydrochloric acid, Tween 80, dipotassium hydrogen phosphate, magnesium chloride, zinc sulfate, calcium chloride, ferric chloride, and distilled water.

Storage conditions and expiration date

Storage conditions: This product should be stored in a dark environment at 2-8 ℃.

Validity period: The unopened original packaging has a validity period of 3 months.

This product is a disposable product.

Probiotic product live count: used for total lactobacillus count in mixed products of lactobacilli and bifidobacteria, as well as selective counting of bifidobacteria.

Selective isolation of bifidobacteria: Adding lithium salt of mupirocin can inhibit miscellaneous bacteria and specifically isolate bifidobacteria from complex samples such as feces and food.

Animal gut microbiota study: used to isolate and count bifidobacteria in fecal samples of cows, minks, and other animals, and evaluate gut microbiota.

Screening and research of probiotic strains: used to screen potential probiotic strains such as Bifidobacterium and Lactobacillus from microbial preparations or samples.

Microbial metabolism research: used as a basic culture medium to study the effects of different carbon sources on the in vitro proliferation and fermentation of bifidobacteria.

Development of selective counting method: as a basis for liquid culture medium, used for quantitative counting of bifidobacteria by MPN method.

Research on Oral Pathogenic Bacteria: The improved formula can be used for metabolomics analysis of oral streptococcus (such as Streptococcus mutans).

Bifidobacterium

Biological characteristics: Gram positive bacilli, with variable shapes such as V-shaped, Y-shaped, forked, curved, or club shaped. No spores, no flagella, no motility; Specialized anaerobic bacteria, sensitive to oxygen; The optimal growth temperature is 37-41 ℃, and the optimal pH is 6.5-7.0; Metabolized through the unique fructose-6-phosphate ketolase (F6PPK) pathway, the main products are acetic acid and lactic acid.

Cultivation characteristics on TPY medium: Colonies are round and raised, with a smooth and moist surface, intact edges, soft texture, and a milky or porcelain white color; It must be cultured under strict anaerobic conditions (such as anaerobic incubators or tanks), otherwise it will not grow; Efficiently utilizing casein peptone, yeast powder, and glucose in the culture medium for rapid proliferation can achieve a high concentration of viable bacteria.