【 Principle 】

Pig stomach digestate, beef extract powder, and yeast extract powder provide nitrogen sources, vitamins, and minerals; Sodium chloride maintains equilibrium osmotic pressure; Glucose provides a carbon source; Phenol red serves as an indicator.

Main components

Composed of pig stomach digestate, beef extract powder, yeast extract powder, sodium chloride, glucose, phenol red, and distilled water.

Storage conditions and expiration date

Storage conditions: This product should be stored in a dark environment at 2-25 ℃.

Validity period: The unopened original packaging has a validity period of 6 months.

This product is a disposable product.

Clinical isolation and culture of respiratory mycoplasma: throat swabs and sputum specimens are cultured to enhance bacterial growth, and Mycoplasma pneumoniae is cultured to assist in the diagnosis of respiratory infections.

Urinary and reproductive tract mycoplasma testing: cultivation of Ureaplasma urealyticum and Mycoplasma hominis from cervical and urethral secretions to diagnose non gonococcal urethritis.

Pre antimicrobial susceptibility testing: After purification and amplification of Mycoplasma, antibiotic sensitivity testing is conducted to guide clinical medication.

Preservation and passage of scientific research strains: Recovery and continuous passage culture of laboratory standard mycoplasma strains to maintain strain activity.

Animal derived Mycoplasma Quarantine: Laboratory animal tissue, cavity swab culture, screening for Mycoplasma infection in animals, and prevention and control of laboratory animal diseases.

Quality control and positive control culture: Microbial laboratory testing with amplification of positive strains for methodological validation and indoor quality control.

Mycoplasma pneumoniae

Biological characteristics: prokaryotic microorganisms without cell walls, with polymorphic morphology (spherical, filamentous), requiring cholesterol and sterols to stabilize the cell membrane; Facultative anaerobic, slow growing, respiratory pathogenic bacteria, causing mycoplasma pneumonia, naturally resistant to antibiotics acting on cell walls.

Cultivation characteristics: slow proliferation in broth, media containing phenol red indicator, decomposition of glucose to produce acid, liquid changes from red to light yellow; Aseptic precipitation, with only mild turbidity and visible fine particle adhesion on the tube wall, and no obvious turbidity to the naked eye.

Ureaplasma urealyticum

Biological characteristics: no cell wall, dependent on exogenous sterols, strictly anaerobic microaerophilic; Colonizing the urinary and reproductive tract can decompose urea, which is a common pathogen of non gonococcal urethritis and can induce infertility and pregnancy infections.

Cultivation characteristics: The broth contains urea and phenol red, which decompose urea to produce alkali. The liquid changes from orange yellow to rose red; Overall clear, with only slight misty turbidity and no obvious precipitation, growing faster than Mycoplasma pneumoniae.

Mycoplasma hominis

Biological characteristics: no cell wall, cholesterol requirement, microaerophilic; Decomposing arginine to produce ammonia, a symbiotic pathogenic bacterium in the urinary and reproductive tract, can cause pelvic and urinary tract infections when the immune system is weakened.

Cultivation characteristics: Using arginine metabolism to produce alkali, phenol red indicator turns red, broth is slightly turbid, small flocculent precipitates can be seen at the bottom, and the growth cycle is between the first two.