【 Principle 】

Peptone and beef extract provide the carbon source, nitrogen source, vitamins, and growth factors required for bacterial growth; Glucose can serve as the energy required for bacterial growth and metabolism; Sodium dihydrogen phosphate plays a role in buffering the pH value of the culture; Sodium sulfite and brilliant green can inhibit the growth of Escherichia coli, Proteus mirabilis, and Gram positive bacteria; Salmonella typhi and other Salmonella bacteria can use glucose to reduce sulfites to sulfides and react with ferrous sulfate to form colonies that appear black. In addition, they can also reduce bismuth ions to metallic bismuth, which gives the colonies a metallic luster and separates Salmonella bacteria.

Main components

Composed of peptone, beef extract, glucose, ferrous sulfate, disodium hydrogen phosphate, brilliant green, bismuth iron citrate, sodium sulfite, agar, and distilled water.

Storage conditions and expiration date

Storage conditions: This product should be stored in a dark environment at 2-8 ℃.

Validity period: The unopened original packaging has a validity period of 6 months.

This product is a disposable product.

Fecal specimen isolation and identification: Directly separate the feces of diarrhea patients by streaking, strongly inhibit the coliform bacteria and positive miscellaneous bacteria, and detect trace amounts of Salmonella.

Separation and purification in food: After adding bacteria, streak BS agar to isolate suspected Salmonella in meat, eggs, dairy products and other foods for national standard testing.

Food and beverage equipment and environmental screening: screening workstations, knives, and container smear samples to identify sources of Salmonella contamination.

Hospital infection monitoring: Collect data from intestinal clinics, waste areas, countertops, and equipment to prevent cross infection within the hospital.

Water detection: detecting domestic sewage, food processing wastewater, etc., to assess the risk of pathogenic bacterial pollution in water bodies.

Purification of positive samples: Single colonies were obtained by transferring the turbid tubes to BS agar for subsequent biochemical and serum confirmation.

Cultivation substrate control: Inoculate standard strains to verify the antibacterial effect and color development ability of bismuth sulfite, and set up a positive and negative control.

Salmonella typhimurium

Biological characteristics: G ⁻ facultative anaerobic bacteria with flagella throughout the body; Not fermenting lactose, glucose produces acid and gas, H ₂ S strongly positive; Human animal co infection, high incidence of meat products and environmental factors, can lead to acute gastroenteritis.

BS agar colony characteristics: large black or gray black colonies with obvious metallic luster; The culture medium around the colony turns black, with brownish black diffusion and precipitation rings.

Salmonella Enteritidis

Biological characteristics: Gram negative bacteria with flagella; Lactose negative, producing H ₂ S, poultry eggs mainly contaminate serotypes, and are prone to group food poisoning.

BS agar colony characteristics: black circular raised colonies, bright metallic luster, and large areas of surrounding culture medium turning black.

Salmonella paratyphoid B

Biological characteristics: Gram negative bacteria can invade the bloodstream and cause typhoid fever; H ₂ S positive, does not use lactose, urease negative.

BS agar colony characteristics: gray black medium colonies, slightly weak metallic luster, and mild blackening around the periphery.