【 Principle 】

Protein peptone and yeast extract provide carbon and ammonia sources as well as trace elements; Lactose is a fermentable sugar; Sodium amide can maintain a balanced osmotic pressure; Gallbladder salt and crystal violet inhibit Gram positive bacteria, particularly Gram positive bacilli and fecal streptococcus; Neutral red is a pH indicator; Agar is a coagulant; Escherichia coli contains glucuronidase that acts on the β - glucuronide bond of 4-methylumbellifery-β - D-glucuronide (MUG), causing hydrolysis. The released 4-methylumbelliferone produces blue white fluorescence under 366nm ultraviolet light. 97% of Escherichia coli, 10% of Salmonella, and a small amount of Shigella have glucuronidase.

Main components

Composed of peptone, yeast extract, sodium chloride, lactose, bile salt No. 3, crystal violet, neutral red, agar MUG、 Composed of distilled water.

Storage conditions and expiration date

Storage conditions: This product should be stored in a dark environment at 2-8 ℃.

Validity period: The unopened original packaging has a validity period of 3 months.

This product is a disposable product.

Special counting of Escherichia coli in food: Plate method counting of Escherichia coli in food and dairy products to evaluate hygiene and safety.

Dual index identification mechanism: Introducing enzyme substrate fluorescence reaction based on VRBA selectivity to achieve one-step identification.

Coliform count: Fermented lactose colonies appear purple red with bile salt precipitation rings around them.

Confirmation of Escherichia coli: It has β - glucuronidase, which decomposes MUG. It shows blue white fluorescence under 366nm ultraviolet light and can be directly distinguished.

Water quality and environmental monitoring: can be used for the detection and counting of coliforms and Escherichia coli in samples such as water and milk.

Improve detection efficiency: No need to transfer BGLB for re fermentation, through real-time fluorescence screening, simplify the process and shorten the time.

Escherichia coli

Biological characteristics: Gram negative short rod-shaped bacteria, facultative anaerobic, ferment lactose to produce acid and gas, and the vast majority of strains have β - glucuronidase.

Cultivation characteristics: The colony is purple red or red, usually with a red bile salt precipitation ring; Under the irradiation of 360-366nm ultraviolet light, the colony emits blue white fluorescence, which is a key characteristic that distinguishes it from other groups of Escherichia coli; The quality control standard requires a growth rate (PR) of ≥ 0.7.